From the Super Mario Wiki, the Mario encyclopedia
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Super Mario series
Super Mario 3D World
Ant Troopers make their first appearance in Super Mario 3D World. They appear uncommonly throughout the game, and are introduced in World 4-1, Ant Trooper Hill. They attack by marching, sometimes emerging from holes or pipes, and keep walking on walls. They cannot be defeated by jumping, but are vulnerable to Ground Pounds, Cat Mario's, Tanooki Mario's and Rosalina's attacks, and projectiles as well. Ant Troopers sometimes carry items on their backs, mainly coins and Super Bells.
Big Ant Troopers are an Ant Trooper subspecies. As their name implies, they are larger than common Ant Troopers, and are also rarer. These cannot be defeated by any attack.
Horned Ant Troopers are basically Ant Troopers wearing an orange helmet with a spike over their body. The helmets prevent their heads from being stomped, making them very similar to Spike Tops, but only if the helmet is facing upward; if they are walking along a vertical surface, they become safe to jump on.
Super Mario Maker 2
Ant Troopers appear as course elements in Super Mario Maker 2 in the Super Mario 3D World level style. Big Ant Troopers and Horned Ant Troopers return as well. When given wings, an Ant Trooper flies in a horizontal line, switching directions when it hits a wall. When given a parachute, an Ant Trooper glides to the ground while flailing its feet. Ant Troopers can be defeated with a Super Star or Builder Mario’s hammer.
Captain Toad: Treasure Tracker
Ant Troopers return in Captain Toad: Treasure Tracker, behaving like they do in Super Mario 3D World. While interacting with them using the pointer or touch screen, they do not stop like regular enemies. If pointed at for enough time, they reward the player with one coin.
Mario & Luigi: Paper Jam
In Mario & Luigi: Paper Jam, Ant Troopers appear alongside Horned Ant Troopers in battle in the Twinsy Tropics Dungeon. If Horned Ant Troopers take enough damage, they lose their spike helmets, turning them into normal Ant Troopers and lowering their defense. While in this state, they attack the Mario Bros. by dashing into them instead.
Mario Tennis Aces
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