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In The Super Mario Bros. Super Show! live-action segment Lost Dog, Pam, having lost her dog, Ike, decided to ask for help from Mario and Luigi in finding Ike. Bursting into Mario and Luigi's apartment, Pam, hysterical and sobbing, told Mario and Luigi of Ike's disappearance. Giving Pam his handkerchief to loudly blow her nose, Luigi told her he and Mario would help her search for Ike. Pam then gives the handkerchief back to Luigi, and says that she is starting to feel better and that she has "always depended on the kindness of strangers", going on to add that Mario and Luigi certainly are strange enough.
Later, as Mario searched for Ike with the periscope of Mario Brothers Plumbing, Luigi tended to Pam, who was eating some pizza, which she said was fine, but that it reminded her of Ike. After sobbing some more thinking of Ike, who Mario said he didn't see, Pam, as well as Mario, rushed to a nearby, ringing telephone, which Luigi answered. As Luigi talked on the telephone, he motioned to Pam that the called wasn't calling to say they spotted Ike, which led to Pam crying some more with Mario comforting her.
Before he finished talking on the telephone, Luigi told the caller, Reilly, that he wouldn't miss his Birdy Lovers meeting on the weekend; as Luigi finished his call, Mario, having heard Luigi's comment about the Birdy Lovers, said that if Luigi could dog call as good as he could bird call, he could help Pam find Ike much quicker. As Luigi mulled over Mario's comment about dog calling, Pam began to cry once again, saying that Ike used to call dogs all the time.
Later, Pam, having heard Luigi's plan to try and call Ike by using dog calls, asked him if he believed his plan would actually work. Reassuring Pam, Luigi quoted the book he was holding, "Hanson's Guide to Doggie Dialogue", saying that if he could replicate the noise Ike's breed of dog made, he could make Ike come running to Mario Brothers Plumbing. Before he tried to call Ike though, Luigi decided to test his dog calling, only to succeed in making the noise of a sheep and a pig instead of a dog.
Claiming that there was something wrong with his throat, Luigi began to try and clear it as Mario asked Pam what kind of dog Ike was anyway. Pam told Mario that Ike was part poodle, part Basset Hound, a little bit of country and rock and roll. After learning this from Pam, Mario asked Luigi if he could call mixed dogs like Ike, to which Luigi replied that he was unsure. Deciding to call Ike anyways, Luigi made an extremely bizarre noise which, surprisingly, managed to lure Ike to Mario Brothers Plumbing.
Overjoyed at having found Ike, Pam picked him up and thanked Luigi for having helped her find him, saying that Luigi was a dog genius.