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Near the beginning of the film, Scapelli shows up at a New York University dig site, demanding that they leave so that his business can continue construction, as one of Scapelli's projects had originally been halted to allow the dig. According to Daisy, Scapelli's workers have been harassing them in order to force them to stop. While he is negotiating with Daisy, he threatens the project, which causes Daisy to leave to call the university for more security. Later, Scapelli's workers are at the dig site, sabotaging the project by breaking several pipes.
Later, Iggy and Spike tell Mario that a human has accidentally broken the wall between dimensions, making it easier for him to invade Earth. Mario reasons that it must have been Anthony Scapelli and his employees. While Koopa is on the verge of invading, Scapelli is present at the dig site when Koopa's Tower merges with the World Trade Center. Afterward, when Koopa and Mario arrive, Koopa attempts to blast Mario with a Devolution Gun but instead hits Scapelli, de-evolving him into a chimp.