Super Mario Bros. film
Early in the movie, Lena is introduced as Koopa's significant other and loyal assistant residing in Koopa's Tower. Upon Daisy being taken into Koopa's Tower by Iggy and Spike, she begins to grow jealousy and resentment, fearing that Koopa is looking to replace her with Daisy. Nevertheless, she follows Koopa's pursuits of obtaining the meteorite piece to merge the dimensions. She removes Daisy from the Goomba Barracks, and takes her to her own personal room up on Koopa's Tower. Lena mentions said room used to be her room before her "promotion". There, she informs Daisy that Daisy's mother is dead and that Koopa needs Daisy to personally merge the dimensions herself. Lena expresses that Koopa believes only Daisy is capable of doing it successfully, but implies she is skeptical of this. Lena is alerted by the Hat Check Girl at the Boom Boom Bar that Mario and Luigi are at the bar, and she arrives with a group of Goombas. Spike and Iggy are arrested by the Goombas, and after Mario and Luigi lose the meteorite piece in their attempts to thwart the Goombas, she successfully picks it up.
Lena attempts to bring the meteorite piece to Koopa, who is angry and distracted, and blows her off. Upset by this, she decides to carry out the mission without Koopa, with intentions to invade Earth for herself. She attempts to kill Daisy, but is thwarted by the royal pet, Yoshi. Daisy runs away as Yoshi pulls Lena towards him with his tongue, and she stabs Yoshi with a knife and follows after Daisy. Koopa learns that Lena secretly made off with the meteorite, and with his orders, Sergeant Simon arrests Lena and confiscates the piece. Sergeant Simon turns over the meteorite piece to Koopa.
During Koopa's battle with Mario, Koopa loses the meteorite piece that Lena secretly grabs while leaning over a railing. The railing gives away and she falls onto wiring below, which electrocutes her. She survives the electrocution, but it affects her hair, spiking it up and giving her a gray streak. A severely burnt Lena laughs maniacally, having successfully confiscated the meteorite piece and runs into the meteorite chamber to merge the dimensions. Daisy and Luigi run after her and warn her not to. She inserts the meteorite into the meteorite chamber and the two dimensions of Dinohattan and Earth begin to merge. The act of this creates a massive power surge that vaporizes Lena, with the blast shooting her skeletal remains into a wall. The skeleton becomes embedded into the meteorite chamber's cavern wall, and Daisy realizes that Koopa was right; only Daisy herself can withstand the force of the meteorite.