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Mario Bros. may refer to:
- Mario Bros., the arcade game released before the Super Mario Bros. NES title.
- Mario Bros., the series of games based on the arcade title.
- Mario Bros., the Game & Watch title that featured Mario and Luigi working together in a factory. It was later re-created for Game & Watch Gallery 3 and Game & Watch Gallery 4.
- Mario Bros., a minigame remake of the arcade game included in every installment of the Super Mario Advance series and Mario & Luigi: Superstar Saga.
- Mario Bros., a stage in Super Smash Bros. Brawl and Super Smash Bros. Ultimate.
- Mario Brothers, a microgame in WarioWare: Twisted!
- Mario Bros., a microgame in WarioWare: D.I.Y. Showcase.
- Mario Bros., a microgame in WarioWare Gold.
- Mario Bros., a microgame in WarioWare: Move It!
- Mario and Luigi, who are sometimes collectively referred to as the Mario Bros.
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