Mario Bros. (stage)
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In the debut game, the sewers are the only location in the entire game, much like many other 2D platforming games. Mario and Luigi are called into service after reports of enemies such as Shellcreepers, Sidesteppers, Fighter Flies and Freezies appearing in the sewers of Brooklyn, New York. Coins and POW Blocks can also be found in the sewers. In the remakes (more specifically, the Super Mario Advance series and Mario & Luigi: Superstar Saga), the Shellcreepers were replaced with Spinies, and Bowser also makes an appearance as an obstacle in one of the variants of the stage.
In Mario Power Tennis, the stage appears in the form as the Mario Classic Court. Shellcreepers, Crabs, Fighter Flies and Freezies occasionally appear on the court. They will block a player who walks into them, and they will be unable to move forward. However, hitting the POW Block that appears on the net will flip the enemies on that player's side of the court, allowing them to be defeated just by walking into them, akin to the original Mario Bros. The enemies can even get faster if left undefeated and allowed to flip back over.
In WarioWare: Twisted!, the stage made an appearance in one of 9-Volt's microgames, Mario Brothers. In it, there is either a giant green or red Koopa Troopa, or a Buzzy Beetle that needs to be shaken out of its shell before Mario and Luigi kick it.
In WarioWare: D.I.Y. Showcase, a similar stage appears in one of 18-Volt's microgames, Mario Bros.. In it, the player must flip the enemies on screen (Shellcreepers and Sidesteppers) by making Mario jump under them by clicking at him. While the Shellcreeper must be hit one time, Sidesteppers must be hit two times. If the player doesn't hit all the enemies, they will lose.
The Mario Bros. stage makes a cameo in Tetris DS, where it appears in the background of the Nintendo DS's top screen if the player chooses any mode besides Records.
Super Smash Bros. series
Mario Bros. is included in Super Smash Bros. Brawl as an unlockable stage and Super Smash Bros. Ultimate as a starting stage. It is a recreation of how the location appeared in the original game and features elements from that game as well, such as enemies and POW Blocks. This is notably the first time that the location from Mario Bros. was called by a name. This is where the unlock match for R.O.B. takes place.
In the Coin Battle mode, the first stage's beginning is very similar to the Mario Bros. stage. Characters can still walk to the left and appear at the right, and vice versa, just like the original stage. The only differences in this area are the design, the POW Block being on a platform instead of floating in mid-air, and coins, ? Blocks and Koopa Troopas have been added to the area. In order to continue the stage, the characters must enter the pipe at the top-center of the area.