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A single Piranha Bean is one of the bosses in Mario & Luigi: Superstar Saga found near Beanbean Castle Town. It must be fought so the Mario Bros. can get a Bean Fruit, which allows them to get the Beanstar piece in the Yoshi Theater, and Luigi must be separated from Mario to fight it. While looking for Bean Fruits, Luigi sees the Piranha Bean eat one, and must to fight it so he can get it back. Upon defeating it, Luigi discovers that Prince Peasley was also swallowed by the Piranha Bean, and was shrunk to a very small size.
During the battle, the Piranha Bean shoots small fireballs out of its mouth at Luigi, and can do so from various spots on the battlefield, which makes its attacks slightly harder to dodge. The Piranha Bean can also shoot the miniaturized Peasley at Luigi as well. It takes critical damage from Thunderhand.
In Mario & Luigi: Superstar Saga + Bowser's Minions, alongside the single Piranha Bean reappearing with the same role as in the original game, multiple Piranha Beans are found in the Minion Quest: The Search for Bowser mode as enemies. They are first encountered in the level Hunger Pains, where one of them bites Captain Goomba at the beginning of the level. They are Ranged troopers, and attack by spitting fireballs at their enemies. Their special attack is Spitfire Fury, which allows them to spit multiple fireballs at a targeted enemy. After the level, Captain Goomba finds a Piranha Bean watching him; after spotting Peasley (whom he had a rather unpleasant conversation with at the start of the level), he directs the Piranha Bean at Peasley, telling it how tasty he looks and prompting the Piranha to eat the Prince.
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Mario & Luigi: Superstar Saga / Mario & Luigi: Superstar Saga + Bowser's Minions
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