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The Border Bros. are the Hammer Bros. that guard the Beanbean Borderlands in Mario & Luigi: Superstar Saga. In order to pass into the Beanbean territory, Mario and Luigi must complete their game of Border Jump, which is basically jumping across a rope.
The player must avoid hitting the rope three times with either character or they will have to restart the challenge. After beating them, Mario and Luigi have the option to come back and challenge them again, but the game is harder every time the brothers beat it. Replaying Border Jump earns them more Hee Beans.
One of them wears a red flag atop his helmet while the other wears a blue one; although the Border Bros.' names are unknown, it is confirmed that the one clad in the blue-flagged helmet is the younger of them. Also, the Border Bros. seem to have known Mario and Luigi from somewhere before their encounter at the Beanbean Borderlands.