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Chuckleroot's granddaughter is an anthropomorphic gnarled tree found in Mario & Luigi: Superstar Saga and Mario & Luigi: Superstar Saga + Bowser's Minions. Like her grandfather, she is found in Chucklehuck Woods. When Luigi enters her private area, she chastises him for entering without her permission, but decides to teach him about finding beans underground since he is in the area. She is not seen again after that.
In Mario & Luigi: Superstar Saga + Bowser's Minions, Chuckleroot's granddaughter is pink with purple stripes, and has a smaller nose.