This town is the first town in the Mario franchise to have an operating actual hospital in the Little Fungitown Embassy. A Toad doctor works at said hospital, aided by Psycho Kamek (replaced by Dr. Toadley in Mario & Luigi: Superstar Saga + Bowser's Minions). The town also has an arcade, and all the various shops and residences resemble mushrooms, much like Toad Houses in other games. Hidden away in the southern part of town, Warp Pipe #7 (#9 in the remake) can be used to traverse to Little Fungitown without having to cross the treacherous Teehee Valley.
During the game's events, Mario and Luigi beat the high score in the Star 'Stache Smash minigame and win an Invincishroom (revealed in Minion Quest: The Search for Bowser to actually be a misidentified Poison Mushroom) as a prize. Mario eats said mushroom, causing him to get Bean Fever, which is then diagnosed by the Toad doctor. Psycho Kamek (or Dr. Toadley in the remake) help hypnotize Luigi into believing he is Mario, so he can gain bravery to retrieve the cure for Mario's Bean Fever: Crabbie Grass.
The barrelminigame is here too, which is available after the S.S. Chuckola sinks. Invisibleblocks are here containing Hoo Beans. When Luigi gets out of Guffawha Ruins, he comes out of a pipe in a top corner of Little Fungitown. Popple is also eventually sentenced to community service in Little Fungitown after being launched out of the depths of Teehee Valley by Birdo.
In Minion Quest: The Search for Bowser in Mario & Luigi: Superstar Saga + Bowser's Minions, Little Fungitown is seen in the mission Toad Rescue, where Morton Koopa Jr. and a group of Fire Bros are found attacking the town. The minions must then battle Morton.
* Exclusive to Mario & Luigi: Superstar Saga. • ** Exclusive to Mario & Luigi: Superstar Saga + Bowser's Minions. † Enemy, boss, or stat exclusive to the main game. • †† Enemy, boss, or stat exclusive to Minion Quest: The Search for Bowser. • ††† Enemy variants differ in Mario & Luigi: Superstar Saga + Bowser's Minions.