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The Biffidus is a member of a tribe-like group of Biffidus, who act as antibodies within Bowser's body, and appear to assist the parasite worm Durmite. They are rounded in shape and carry spears, which they use to attack. During the events of Mario & Luigi: Bowser's Inside Story, Bowser ate a huge carrot which, unknown to him at the time, contained Durmite. The Biffidus assisted Durmite in battle, by trying to jab Mario or Luigi with their spears. However, after taking much damage, Durmite would eat the Biffidus, then regurgitate their spears at Mario or Luigi, which they must jump over the flying Biffidus. However, the Biffidus were no match for Mario and Luigi, and were ultimately defeated, along with Durmite. In the battle with Durmite X, Biffidus Xs appear instead of regular Biffiduses.
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