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A Biffidus from Mario & Luigi: Bowser's Inside Story + Bowser Jr.'s Journey.
First appearance Mario & Luigi: Bowser's Inside Story (2009)
Latest appearance Mario & Luigi: Bowser's Inside Story + Bowser Jr.'s Journey (2018)

The Biffidus is an enemy that appears in Mario & Luigi: Bowser's Inside Story and Mario & Luigi: Bowser's Inside Story + Bowser Jr.'s Journey. They reside in Bowser's body and appear to act as antibodies, attacking any foreign entities that they encounter. Biffidus appears as a small soldier, with a round body, stubby arms and reddish-pink feet. Their face appears pitch-black, save for a pair of white eyes. Each Biffidus wields a metallic spear and a brown shield.

A stronger variant known as Biffidus X appears during the battle against Durmite X in the Gauntlet.


Shortly after eating the giga-carrot, Bowser comes down with a painful stomachache and demands that Chippy (who is actually Starlow) investigate immediately. Mario, Luigi and Starlow go to the Nerve Cluster to find the source of the pain, and immediately encounter Durmite, who breaks through a wall of bone. As Durmite slowly approaches the group, a trio of Biffidus appear to menace them only to be eaten by Durmite, causing it to grow larger. Mario suggests they have to defeat Durmite in order to relieve Bowser's pain, and the Mario Bros. face it in battle.

After defeating Durmite, Mario and Luigi enter the next room and are ambushed by an army of Biffidus that cling to the ceiling. The Biffidus drill into the ground, causing the platform the Mario Bros. are standing on to come loose and fall into the pit below, where the Mario Bros. encounter Toadbert. Toadbert explains that the Biffidus attacked him as well, speculating that they attack anything foreign they find within Bowser's body.


Every few turns or so, up to three Biffidus appear to assist Durmite during its boss fight. They attack by pointing their spears forward and running at the Mario Bros., who can dodge and counter by jumping. On Durmite's turns, it may eat all the Biffidus and spit their spears at Mario or Luigi, which they must jump over; each spear points towards its target. The Biffidus will stand clear of Durmite while it uses its other attacks; in Mario & Luigi: Bowser's Inside Story + Bowser Jr.'s Journey, they run off-screen for that move's duration.

If any Biffidus are present when Durmite is defeated, they jab their spears in its direction as the animation plays, with the explosion of stars blowing them away.


Mario & Luigi: Bowser's Inside Story[edit]

Mario & Luigi: Bowser's Inside Story enemy
M&LBIS Biffidus.png HP 10 (15) POW 18 (45) DEF 16 (24) SPEED 50 (75)
Coins 0 (0) Level 7 Fire Double Burn? Normal
Role Support Battled by Mario and Luigi Location(s) Nerve Cluster Dizzy? Normal
Stat down? Normal KO? Immune Experience 0 Item drop Syrup Jar - 5%
Notice: The second set of numbers next to the enemy's HP, POW, DEF, SPEED and Coins are stat increases from the Challenge Medal accessory; a 50% increase for HP, DEF, SPEED and Coins earned, and a 150% increase for POW.

Mario & Luigi: Bowser's Inside Story + Bowser Jr.'s Journey[edit]

Mario & Luigi: Bowser's Inside Story + Bowser Jr.'s Journey enemy
Biffidus HP 10 POW 18 DEF 14 SPEED 50 Experience 0
Fire Critical Jump Normal Hammer Normal Battled by Mario & Luigi Coins 0
Burn 2x Dizzy 1x Stat down 1x Speed down 1x Item drop Syrup Jar - 30%
Nut - 10%
Level           7 Location(s) Nerve Cluster

Names in other languages[edit]

Language Name Meaning
Japanese ビフィーズ人
Bifīzu Jin
From a partial elongation of「ビフィズス菌」(bifizusu-kin, bifidobacteria) and「人」(jin, person)
They are the only enemy in the game to have a kanji in their name during battle. Other enemies with a kanji name revert to hiragana during battle.

French (NOA) Enzymo
French (NOE) Bifigus
Pun on "bifidus" and "gus", a slang word equivalent to "gizmo"
German Oxygardist
From "Oxygen" and "gardist" (guard)
Italian Bifidus
Spanish (NOA) Enzimo
Masculine name form of "enzima" (enzyme)
Spanish (NOE) Bífido