From the Super Mario Wiki
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Small Spiders are encountered in Fungi Forest only at night. They are encountered by Tiny Kong in the lair of the Giant Spider inside a barn that Tiny gets to by using Mini-Monkey. They sometimes come down and try to attack Tiny. These Small Spiders take a number of hits before being defeated, but they will be defeated in one hit by the shockwave attack. When the Giant Spider is defeated, it transforms into a Small Spider, which can be defeated in one hit.