From the Super Mario Wiki, the Mario encyclopedia
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Small Spiders (alternatively known as Spiders) are one-eyed, bluish-purple spiders and enemies in Donkey Kong 64. They are only encountered in Fungi Forest at night, during the mini-boss fight against their larger counterpart, the Giant Spider.
The Giant Spider summons three Small Spiders to attack Tiny at the start of the battle. They can be defeated from a few hits, or instantly from the shockwave attack. For each time that Tiny attacks the Giant Spider, it summons three more Small Spiders after Tiny. Eventually, after five hits, the Giant Spider transforms into a Small Spider, and can be defeated just like one.