From the Super Mario Wiki
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The game is filled with a total of forty Banana Medals, with a total of five medals per level. During the levels, the five Kongs can get these medals by collecting seventy-five of their own bananas. Once the Banana Medal is earned, it can never be obtained again. In the game's final level, Hideout Helm, the rules to getting Banana Medals are much different, as the heroes will need to complete their own bonus levels to get it.