In Mario is Missing!, Bowser and the Koopalings travel to Antarctica and build a fortress there. Bowser plans to steal and sell various artifacts, so he can use the money to buy hair dryers from Hafta Havit Mail-Order to melt Antarctica, flooding the Earth.
While it does not appear in actual gameplay, Antarctica is visible alongside Earth's other continents on the online racing menus for Mario Kart Wii and Mario Kart 7.