From the Super Mario Wiki, the Mario encyclopedia
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Chime Notes are objects that appear in Yoshi Topsy-Turvy. They are multicolored objects that seem to take the appearance of a toy xylophone. They can appear either red, yellow, green, blue, or purple. When Yoshi rolls a Chime Ball over them, they will play a tune, such as Twinkle, Twinkle, Little Star, which will create music notes that can be used as platforms for Yoshi.