From the Super Mario Wiki
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In Dance Dance Revolution: Mario Mix, as the name implies, the item resembles a large heart in a container. It can be bought at any shop run by a Lakitu in the Story Mode of the game. Mario or Luigi can purchase one for 170 coins. Up to three Big Hearts can be carried at a time. If Mario or Luigi have one in their possession and use it before a dance, the dance meter completely fills up once it is empty. Even if the Big Heart is not necessary, it still disappears from the item inventory. If it is not used, they keep the Big Heart in their possession.