Pookie's only appearance in the comic series was the story "Bowser Knows Best." Pookie can be seen cowering in his cage on the first page of the comic, while the Koopalings are deciding what to get their father, Bowser, for Father's Day. Roy suggests that simply beating Pookie would be a suitable present for Bowser.
Roy later accidentally drops and breaks Pookie's cage when Wendy forcibly drags him away to help her and the others capture Princess Toadstool. Free of his cage, Pookie hops away, and he can be glimpsed a few times wandering through Bowser's Castle.
Pookie later appears on the last page of the story, while Bowser is distracted reading a calendar that Lemmy had written (rather poorly) "Be Kind to Turtle Kid Day" (a fake holiday made up by Princess Toadstool) on. Pookie manages to escape his owners by jumping out a window, just as Roy realizes he is missing.
|Bowser, forms, and counterparts|
|Alarm Koopa • Baby Bowser • Black Bowser • Blockhead Bowser • Bowcien • Bowser • Bowletta • Castle Bowser • Dreamy Bowser • Dry Bowser • Fury Bowser • Giant Bowser • Giga Bowser • King Koopa's alter egos • King Tetris • Koopa 3 • Magma Bowser • MegaDragonBowser • Meowser • Paper Bowser • President Koopa • Shiny RoboBowser • Shrowser|
|Current in-game relatives|
|Dry Bowser • Bowser Jr. • Paper Bowser Jr.|
|Former in-game relatives|
|Bowser's Brother • Koopa Kid • Koopalings (Larry Koopa • Morton Koopa Jr. • Wendy O. Koopa • Iggy Koopa • Roy Koopa • Lemmy Koopa • Ludwig von Koopa)|
|Bowser's cousin • Bowser's father • Bowser's mother • Bowser's sister • Enma Daiō • King Koopa's great-great grandkoop • Poopa La Koopa|
|Mechawiggler • Pookie • Ratso|
|President Koopa's relatives|
|Iggy • Spike|