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The Bugle is one of the nine items in Wario Land 4 that can be bought at the Item Shop at a price of six medals (depending on the game mode, it can cost more). All items in the Item Shop can be purchased to assist Wario in defeating a boss by doing pre-damage at the battle's start. The Bugle varies from a real life bugle, since it has multicolored stripes on it. When purchased, the Item Shopkeeper will follow the player to the boss room, carrying the Bugle. She will then start playing the Bugle and many music notes will come out and inflict an average amount of damage to the boss. The Item Shopkeeper will then exit the room, leaving Wario to fight a slightly weaker boss.
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