From the Super Mario Wiki, the Mario encyclopedia
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There are two prisons in Bowser's Castle used to lock the people at Princess Peach's party who failed to escape (excluding one which isn't locked by a Prison Key). The first one is to the east in the first center of the castle and imprisons three Toads and a Dryite. The second one is to the west in the second center of the castle and imprisons three Toads and a Bumpty. When Mario sees a prison, he can optionally defeat the Koopatrol outside it and open the prison door. But if he does so, the prisoners inside will say that it is safer for them to stay there and wish Mario good luck defeating Bowser. One Toad will also make a makeshift Toad House with the slate in the prison.
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