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At the beginning of her story, Penny is shown announcing the completion of a new potion, which she calls Tonally Transformative Elixir. Dr. Crygor appears, and Penny informs him of her plans to use the elixir to enhance her voice and become a pop star. She then tests the elixir on Dr. Crygor, and although it successfully makes his voice high-pitched, it has a terrible taste, and also puts him in a condition of extreme stomach pain. Remembering her Super-Special Tummy Tonic, Penny gives it to her grandfather to use as an antidote for the elixir.
The tonic's results are successful, and Dr. Crygor's stomach pains subside. As a suggestion, he advises Penny to work on the painful side effects the next time she works on her elixir. He then asks when the side effects on his voice are supposed to wear off. Penny does not respond, suggesting that the effects are permanent.
Penny's Lab makes a minor appearance in the intro of Penny's Mix in WarioWare: Get It Together!, where Penny can be seen inventing the Jet Tank One alongside Doris 1. This time, the lab is bigger and less colorful, and there is also a red umbrella and a realistic-looking satellite on the roof. The interior of Penny's Lab is also realistic-looking.