Like Red Wharf and Ford Knocks, Jetty Jitters takes place on a series of boardwalks overhanging a shallow river. Eventually Dixie and Kiddy must climb across several horizontal ropes in order to cross the wide gaps between the wharves. Most ropes have a Klasp hanging from them, moving back and forth in a fixed pattern. Klasps are the main enemy in the level. To avoid Klasps, the Kongs can jump over them or swim in the river below. Many Lurchins are in the river below, and the Kongs must avoid them. On the boardwalks, the Kongs encounter Kobbles and Sneeks.
Jetty Jitters is the game's equivalent to the Donkey Kong Country 3: Dixie Kong's Double Trouble! level, Kreeping Klasps, because both levels share similar features. Jetty Jitters is among the few levels shown on the title screen's gameplay demo.
At the beginning of the level, the heroes have to walk down a short, slanted wharf and cross a small gap. Soon, they reach a long tightrope. After dodging all the Klasps on it, they need to jump to the letter K and the next rope. Once the group jumps off one last tightrope, they have to get back on the wharves and head under a small roof-like structure. Eventually, the monkeys walk up some more slanted paths and need to climb over a long tightrope. Once they get over the tightrope, they have to roll into a nearby gap to find the letter O, and then cross another small pit to find the Star Barrel.
About halfway through the level, the Kongs find another tightrope to climb across. Once they find the third tightrope in that area, they will be able to collect to Bear Coins, as well as the letter N. After this, they need to drop down onto another rope and make their way back to solid ground. There, they have to walk under another roof, defeating Kobbles, and cross a small gap to find another roof towering above the wharves. Beating some Kobbles again, they find the letter G and have to swim through a small watery area. Though most parts of the water in this level are filled with Lurchins, this part only has one foe in it, making the monkeys journey through this area a little easier. Once out of the water, the apes need to walk up another slanted pathway and get over more boardwalks to find another gap. After crossing it, the heroes walk up a slanted pathway again to find a flagpole. One touch of the flagpole ends the level.
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