Tropical Tightropes takes place in a rainforest setting. The Konveyor Ropes reappear from an earlier level, Jungle Jeopardy. Dixie and Kiddy slide across the tightropes to progress through the level. Many Buzzes appear around the ropes. Along the way, the Kongs must also use Barrel Cannons to go across gaps. It is comparable to Konveyor Rope Klash, a level from Donkey Kong Country 3: Dixie Kong's Double Trouble!
At the beginning of the level, the heroes will have to head down a long path, defeating a few enemies, such as Sneeks. Upon entering a nearby barrel, the group will be shot over a large abyss and land on the other end of it, where they will have to avoid some more enemies to find another barrel. Going into this barrel, they will be shot onto a moving tightrope. It will pull them towards some Buzzes, so the heroes will need to jump over each foe and hop carefully to the separate tightropes. After grabbing the letter O, the Kongs will need to enter another barrel and be shot into the Star Barrel on flat land.
About halfway through the level, the monkeys will have to trudge through the jungle and cross a small gap to find another barrel. It will shoot them to two parallel tightropes. The group should hop back and fourth to each rope to dodge a few Buzzes, then hop to the next tightrope connected to another tree. Following a small banana, they will have to plummet back to the ground and travel east. After avoiding some enemies, including a Bazuka, the apes will notice another barrel. Hopping into it, they will be blasted to more parallel ropes. Continually jumping back and fourth to each tightrope, they will, again, have to dodge several Buzzes. At the end of the final tightrope of the area, the Kongs will find the letter N. From there, they should fall off of the rope and head down the path. Nearby, they will see a barrel. It will shoot them onto yet another set of parallel tightropes. Jumping back and fourth between the rope, as before, they will need to watch out for Buzzes. At the end of these tightropes, they will have to jump into another barrel and find the flagpole. If they touch it, they will be lead to the next level.
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