From the Super Mario Wiki
Five-Second Rule is one of Dribble and Spitz's microgames in WarioWare: Twisted! The name comes from the term "five second rule," which refers to picking up food off the ground in under five seconds, making it safe to eat.
The game's purpose is to protect a candy from ants. To do so, the player has to move the hand and swat them. If done so, the game is won. However, if an ant reaches the candy, the player loses.