From the Super Mario Wiki, the Mario encyclopedia
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Humans are one of the many sapient species that appear throughout the Mario franchise. They are not nearly as numerous as other species, such as Toads, Piantas, Goombas, and Koopa Troopas, with the exception of the Metro Kingdom.
Some instances of humans are implied. For example, the skeletons of what appear to be humans can be found shackled in chains in several of the sunken ships in the area Gloomy Galleon in the game Donkey Kong 64.
In New Donk City from Super Mario Odyssey, realistic humans known as New Donkers are the local species, forming most of the city's population. With the exception of New Donkers, most humans in the Mario franchise are designed either cartoonishly (like Mario, Luigi, and Princess Peach) or have a more anime look to them (the various NPCs in older Mario Golf and Mario Tennis games).