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Mario starts off on a platform next to some spikes. As Mario makes his way through the level, Donkey Kong constantly throws Poison Mushrooms down from his perch, which Mario must avoid. The platform on the left leads to a set of beanstalks, with a moving platform that leads to another beanstalk and the next section. This section contains some fruit and a Snapjaw, with a platform close to where Pauline is standing; reaching her will clear the level, with Donkey Kong taking her to the next world.
In the cutscene that follows, Mario comes towards Donkey Kong and Donkey Kong Jr. with a hammer, but the pair are on higher ground; Mario throws his hammer upwards, climbs the ladder, and reobtains it, with Donkey Kong Jr. and Donkey Kong running away as he gives chase.