From the Super Mario Wiki
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The Paintbrush is an item in the SNES and PC releases of Mario's Time Machine. It used to belong to Michelangelo Buonarroti while he apprenticed under Domenico Ghirlandaio and his brothers, but after Michelangelo left to pursue sculpting, it was taken by one of his fellow apprentices. This apprentice later gives the Paintbrush to a time-traveling Mario in Florence after Mario stops by for a chat. Mario can then bring it to Raphael Sanzio, who had previously mentioned that he needed a new paintbrush. Once received, Raphael admits to attempting to give Mario the "brush-off", but still decides to help Mario with finding out the owner of the Chisel in Mario's possession.