From the Super Mario Wiki, the Mario encyclopedia
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Room 503 is the hotel room that Luigi stays in at the beginning of the game. At the start of the game, like the rest of the hotel, the room is brightly lit up and colorful. There is a desk with a chair, a wardrobe, a bed, and a table with two arm chairs. Once Luigi talks to Polterpup, a cutscene will ensue where Luigi grabs a flashlight and a book, lies down on his bed, and then immediately falls asleep.
Once night falls, the Last Resort becomes dark and scary. Fog appears on the floor. The bright balloons get replaced with balloons with scary faces. The gift basket gets replaced with a basket of rotting fish. Gargoyle statues appear outside the windows, and the thumbs up and the smiley face that appeared before get replaced with a hand with sharp claws and a jack-in-the-box, respectively. There are two coins in the drawer to the left of the bed.
If Luigi goes to the northwest side of the room, outside the window, a crow will fly away. A few seconds later, a bunch more crows will fly away, causing Luigi to get scared.
When Luigi gets back here a second time, the door to this room will be invisible. To reveal it, Luigi must use the Dark-Light Device
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