From the Super Mario Wiki
“You make me so mad I could spit!”
Ptooies are a type of Piranha Plant that have the ability to walk, although some of them are stationed in pipes. These plants have appeared in Super Mario Bros. 3, and Super Princess Peach. They can also shoot spiky balls out of their mouth.
 Super Mario series
 Super Mario Bros. 3
Ptooies make their debut in Super Mario Bros. 3 as regular enemies. A Ptooie can be identified by the spiked ball it suspends in the air over its mouth. Occasionally, Ptooies will spit the spiked ball high into the air in order to inflict damage on passing travelers. As with standard Piranha Plants, Ptooies can be destroyed with fireballs, hammers, Koopa Shells, or Raccoon Mario or Tanooki Mario's tail.
 New Super Mario Bros. Wii
 The Adventures of Super Mario Bros. 3
Ptooies made a few appearances in The Adventures of Super Mario Bros. 3, where they would always attack Mario and/or his friends on sight. They were sometimes used as guns. Unlike in the game, where they were limited to shooting a single spiked ball into the air, Ptooies could shoot a whole barrage of spiked balls in any direction.
Ptooies in the show were capable of speaking in the episode "Never Koop a Koopa", despite the fact that most members of the Piranha Plant family never speak at all.
 Nintendo Adventure Books
Two Ptooies appear in Koopa Capers, presumably brought to Dark Land by Wendy O. Koopa, who needs one to season a potion she intends to create. Encountered by Luigi in a Fortress, the plumber escapes the plants by jumping over them while absentmindedly wondering what they are doing wandering around in a dark and dank fortress, instead of being outside.
 Super Princess Peach
In Super Princess Peach, if Princess Peach uses Rage to pound the ground or simply uses Poundbrella nearby, the ball will be lost, but Ptooie will survive. They will then simply move back and forth slowly until defeated. Even without the ball, Ptooies cannot be stomped.
 Names in other Languages