Sprixie Princesses rule the Sprixie Kingdom and are captured by Bowser at the begining of the game. At the end of every world, the player rescues a Sprixie Princess and it builds a Clear Pipe to the next world. After World Castle, Bowser captures all the Sprixie Princesses again, but they are rescued permanently when the player defeats Meowser in World Bowser.
Sprixies are fairy-like creatures that are ruled by Sprixie Princesses. They appear in many levels and hold binoculars, allowing the player to see the end of the level. They are also found in Sprixie Houses.
Lumas make a minor appearance as non-playable characters. They can be found floating around the map in the bonus worlds. The player cannot interact with them
Plessie is a character that the player can ride across water. The player must tilt to move or accelerate. The player can press to jump. He functions similarly to Dorrie from Super Mario 64 and Manta Ray from Super Mario Galaxy.
Bowser is the main antagonist who invades the Sprixie Kingdom and captures the seven Sprixie Princesses. Bowser drives his Bowser Mobile and attacks by throwing Kick Bombs and fireballs at the player. He is fought by returning the Kick Bombs at him and is defeated after nine hits.
Hisstocrat is a snake-like creature who attacks by emerging from the ground and hurling boulders at the player. He also sends out smaller snakes, some of which have Super Bells on them. To defeat him, the player must stomp on his head three times.
Queen Hisstocrat is a female version of Histocrat. She acts very similarly; her only differences are that she uses fireballs instead of boulders and that she is pink.
Boss Brolder is a large boulder that attacks by attempting to roll over the player or release flaming rocks. He is defeated by hitting smaller boulders at him three times.
King Ka-Thunk is a metal square with spikes that is attached to the inside of a large Ka-Thunk. He mainly attacks by moving around inside his frame and trying to slam down on the player. To defeat him, the player must stomp his backside three times.
Prince Bully is a large Bully that attacks by ramming into the player and spitting fireballs. He is defeated by being pushed into a Clear Pipe and stomped on after he comes out three times.
Motley Bossblob is a Koopaling-like clown that attacks by gathering large metallic blobs to become a large monster, then attempting to jump on the player. The player must defeat him by stomping on him three times when he releases his blobs.