Papa Mario
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Mario and Luigi's father, presumably Mr. Mario or Papa Mario, is Mario and Luigi's father. He is a wide-set man, similar in frame to Mario. None of his appearances gave him a speaking role and his face has never been seen. At the time of his sons' births, Mario and Luigi's father was living in a small mushroom-shaped house in the Mushroom Kingdom with Mama Mario, though it is not confirmed that they were married. He smoked a pipe while his sons were toddlers, and wore a tucked-in collared shirt during the day, and a nightshirt and slippers to bed. All other aspects of his life, including his current appearance, personality, residence, and relationship with the boys, are unknown.
Appearances
Yoshi's Island series
Mario and Luigi's father first appeared in Super Mario World 2: Yoshi's Island, when he and his supposed wife found Baby Mario and Baby Luigi on their doorstep, after having been delivered by the stork. Unaware that his sons had just been rescued from the Koopa Troop by the Yoshis, Mr. Mario held Luigi aloft (Mama Mario had Mario), and entered the world of parenthood.
However, the babies were not yet out of danger, and were kidnapped once more by Kamek in the middle of the night during the events of Yoshi's Island DS, causing a great commotion in the Mario household. But while Mr. Mario and Mama Mario knew that their children were in peril this time, they were still powerless against the Koopas, and the Yoshis saved the day once again. The stork returned the babies, and although it was not the end of their adventures (as babies or as adults), their father has not make another in-game appearance (not counting the Game Boy Advance port of Yoshi's Island: Super Mario Advance 3).
Nintendo Comics System
Mr. Mario was pictured in the third panel of Family Album "The Early Years", which was released five years before Yoshi's Island, but is set later, when Mario was a toddler. Here, Mario is shown to have meddled with his father's pipe, making it produce bubbles instead of smoke; he is pictured fleeing from from an irate Mr. Mario.
Super Mario Amada series
In the Super Mario Amada series adaption of Momotaro, Mario is actually born from a giant peach that fell from the sky. He is then adopted and raised by his new "parents," an elderly Hammer Bro. couple (who conincidentally were also Princess Peach's grandparents).
Relatives
In the games, Mr. Mario has two sons: Mario and Luigi; and their mother, Mama Mario, is presumed to be his wife. Earlier sources, (such as Family Album "The Early Years" and the game Super Mario Bros.) implied that Mario was older than Luigi, however as of Yoshi's Island, they have been recognized as twins.
The Super Mario Bros. Super Show! introduces many more members of the Mario family. Mr. Mario has a pair of nieces named Marianne and Luigeena, and two nephews called Mario Joe and Luigi Bob. As it is not revealed which sides of the family any of the relatives hail from, it is unknown whether Grandma Mia is Mr. Mario's mother or mother-in-law, and whether Aunt Luigeena is his sister or sister in law. The Adventures of Super Mario Bros. 3 mentions a second sister/sister-in-law named Aunt Maria.
Also, according to the Saturday Supercade's Donkey Kong segment, Pauline is Mario's niece, which would make Mr. Mario her grandfather. However, this contradicts the continuity of the Mario series, in which she is Mario's ex-girlfriend, and thus of no relation to his parents.
