From the Super Mario Wiki
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Barbara is the mascot of Daigasso! Band Brothers, the Japan-exclusive predecessor to Jam with the Band. She is the arrogant owner of the music shop of Waruwaru Town, GB Music, and helps the player throughout the game. She also made several appearances in Mario-related media, most notably as an Assist Trophy in Super Smash Bros. Brawl.
Super Smash Bros. series
Super Smash Bros. Brawl
When Barbara is summoned in a battle via an Assist Trophy, she strums her guitar for a few seconds, releasing sound waves that moderately damages opponents and knocks them back. Her last sound wave is larger and stronger than the other ones.
In addition, a collectible trophy of Barbara can be obtained when it appears at random in the game's single-player modes.
Super Smash Bros. for Nintendo 3DS
Barbara returns in Super Smash Bros. for Nintendo 3DS as a collectible trophy.
Super Smash Bros. Ultimate
Barbara appears in Super Smash Bros. Ultimate as an Ace-class Grab-type primary spirit. It has three support slots and, when used on a fighter, only boosts their power without granting additional effects. The spirit is summonable with a Rouge the Bat, Prince Saruno, and a grab-type core.
Super Mario Maker
Barbara later appears in Super Mario Maker as an add-on Mystery Mushroom costume for Costume Mario. It can be unlocked by clearing the "Barbara in Tomatoland" Event Course, which was released on February 25, 2016.
Barbara appears in a web comic strip posted on April 01, 2016 on Nintendo's Japanese kids website called Ashley Meets Halloween and Barbara and Ashley. In the strip, Barbara hires Ashley to sing at her live club, but she struggles due to her shyness. Barbara watches Ashley and smugly thinks Ashley can make her rich, but soon after this, the witch falls asleep on stage, to Barbara's dismay.
Profiles and statistics
Super Smash Bros. Brawl trophy
Super Smash Bros. for Nintendo 3DS trophy
Super Smash Bros. Ultimate spirit