Mario Bros. may refer to:
- Mario Bros., the arcade game released before the Super Mario Bros. NES title.
- Mario Bros., the Game & Watch title which featured Mario and Luigi working together in a factory. It was later re-created for Game & Watch Gallery 3 and Game & Watch Gallery 4.
- Mario Bros., a stage in Super Smash Bros. Brawl.
- Mario Brothers, a microgame from WarioWare: Twisted!
- Mario Bros., a microgame in WarioWare: D.I.Y. Showcase
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