Chuckleroot
Chuckleroot is a large, gnarled tree that is Wario and Waluigi's Mother. She lives in Chucklehuck Woods. She guards the Mother Chuckalola, Wario and Waluigi's sister, where the Chuckola Reserve is located. During the events of Mario & Luigi: Superstar Saga, the heroes of the Mushroom Kingdom, Mario and Luigi, travel to the forest to find a cure for Queen Bean's stomach illness. Before they can pass however, Chuckleroot asks the plumbers to collect three special Chuckola Fruits as proof of their worthiness. Eventually, Mario and Luigi collect the requested fruit and enter the Chuckola Cola brewery.
Chuckleroot also has an unnamed granddaughter that Mario and Luigi meet during their time in Chucklehuck Woods. She is also a gnarled tree, although smaller than her grandmother and a little bigger than her parents Wario and Waluigi. She teaches Luigi how to obtain beans from the ground.
