Mushroom Land is the second level of Yoshi's Safari. It is located in the Light Realm of Jewelry Land. It can be accessed once Mario and Yoshi complete Grass Land. After Bowser stole the twelve treasured gems of the land, he gave one of them to Ludwig von Koopa, one of his children who then took it with him to Mushroom Land. Mario and Yoshi have to come here in order to collect all of the twelve gems and rescue the kidnapped King Fret and Prince Pine.
Mushroom Land features an autumnal landscape with wide yellow meadows. The level is named after the numerous pink mushrooms that are scattered along the wayside and in the background. Also in the background are some large green hills. Common enemies in Mushroom Land include yellow Koopa Paratroopas, Paragoombas, Nipper Plants, and Podoboos. At one point, Mario and Yoshi encounter a barrage of Green Koopa Shells sliding across the path. Mario has to clear the path with his Super Scope before traversing in order to avoid damage. Aside from that there are no obstacles that need to be jumped over, and no mini bosses to fight.
 Boss Fight: Ludwig von Koopa
At the end of the Mushroom Land level, the player battles Ludwig von Koopa. The Koopaling fights Mario and Yoshi in a large robot modeled after a turtle with a long neck and spiked shell. The Koopaling sits in the machines head. He attacks by firing green blasts out of one of the two cannons on the side of the robot's head. The machine's weak point is a large purple bulge on the top of the shell. After taking enough damage, the mech explodes leaving only its legs on the ground, then Ludwig, still in the head, drops out of the sky as the robot transforms, growing arms where the cannons were previously. This new robot fires from the arms and mouth. Destroying the arms and then shooting the mouth destroys the robot. Ludwig lands on the ground waving a white flag and the level is completed after Mario receives the gem, Ludwig drops.