Aunt Luigeena is the aunt of Mario and Luigi. She is an elderly, cruel, and much-feared woman; she first appeared in The Super Mario Bros. Super Show!.
Near the end of The Super Mario Bros. Super Show! live-action segment "Mama Mia Mario", Aunt Luigeena appears at Mario Brothers Plumbing, apparently to visit Mario and Luigi; shortly after arriving, Aunt Luigeena begins to berate Mario, who is dumbstruck and horrified to see her in his apartment.
Trivia
- Aunt Luigeena may be the namesake of Luigeena, one of Mario and Luigi's cousins from a previous live-action segment of The Super Mario Bros. Super Show! entitled "Marianne and Luigeena".