List of New Super Mario Bros. Wii pre-release and unused content

Beta Elements from the game New Super Mario Bros. Wii.

Propeller Mushroom
The orange Propeller Mushroom was originally a Propeller Suit, a red letter "P"-like suit with a propeller at its top. It's unknown why the makers changed it into a Mushroom.

Level Designs
Many of the platforms were redesigned as many of them were orginally plain in color. There are also differences in mechanics of some of the platforms. There was earlier going to be platforms moving on their own, close by growing and shrinking pipes, but those platforms can now be controlled by players doing something specific.

Fire Toads
Originally the fire Toads had caps which had their respective colors reversed with white spots on them. In the latest screens from the games, the Toads instead have red spots on their caps.

HUD during gameplay
During the early screenshots of the game, the HUD originally appeared transparent during gameplay; additionally, the text appeared very different, with only generic font; possibly as a placeholder. Recent screenshots and videos of the final build show that the HUD is now solid (only turning transparent if a player goes behind it), certain icons were updated or brightened up, and along with the trademark Mario text. Notably, the life box was sized down, while the score and timer stretched out slightly. Also Yellow Toad was aligned after Blue Toad.

Also, when a Yoshi is ridden, a Music Note appears on the upper-right side of the screen. This may have been deleted because that people may intend that the note stands there for no reason

Red Yoshi
Earlier at announce, Nintendo confirmed that a Red Yoshi is ridable (even an artwork of it has been made, saying that it would stay so). However, after a newer trailer, Red Yoshis haven't been seen again in this game, but instead, Pink Yoshis have been added (the artwork for the Red Yoshi has changed to match the current change). It may be possible that the Pink Yoshi replaced the Red one.

Blue Yoshi
The Blue Yoshi was also supposed to be in New Super Mario Bros. Wii ever since the gameplay, a Blue Yoshi is rideable and was confirmed, but it is possible that Light Blue Yoshi replaced Blue Yoshi in the latter.

"Level Clear!"
In the E3 2009 demo, whenever a character completes a stage, the message "Level Clear!" appears. It has since changed to "Course Clear!" for unknown reasons.

Sound Effects
During the E3 2009 demo, the coins had 8-bit sound effects, just as they did in the first New Super Mario Bros., but that was later replaced with 16-bit coin sound effects, the exact same ones from Super Mario World and Super Mario All-Stars.