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 * Aside from its reappearance in Mario Kart 8, arrangements for Rainbow Road's music from Mario Kart 64 can be heard in later titles. Part of Rainbow Road's music from Mario Kart 64 was arranged and used in the music for Rainbow Road in Mario Kart: Double Dash!! and Mario Kart 7. Additionally, part of the music can be heard in World 9's world map theme in New Super Mario Bros. Wii. When first driving the rainbow road in Paper Mario: Color Splash, this music can be heard.
 * All 8-bit sprites have only three colors, but Bowser's sprite from Super Mario Bros. appears to have four. This is because the black background is what gives Bowser his black spiked collars around his wrists. Due to this, using cheat codes so Bowser appears in a non-castle level will look like his hands are separated from his arms. This occurs again in Super Mario Bros. 3 where Bowser's sprite is designed to exploit the black background to give him an outline.
 * Super Mario Strikers is the first game to feature Kenneth W. James who temporarily replaces Scott Burns as the voice of Bowser, a role that would become permanent starting with Super Mario Galaxy. Likewise, Jen Taylor did not voice Princess Peach and Toad or record any new voice clips for the characters, even though the game was released before her departure from Nintendo, making it the only Nintendo GameCube Mario game where she does not voice Peach and Toad.
 * In New Super Mario Bros. Wii and all subsequent New Super Mario Bros. games, when a mini character enters a mini Warp Pipe, they will exit out of a normal-sized one. This is done to prevent glitching in multiplayer modes, since only one player needs to be mini to get all of the players into the pipe, and the game may glitch if normal-sized players exited through a mini pipe.