N64 Rainbow Road

Rainbow Road is the final track in Mario Kart 64 and the last course of the Special Cup. It is the longest race course in Mario Kart 64 at 2,000 meters (2 kilometres) and requiring around two minutes per lap, making it the longest in the whole series. The track differs from the other Rainbow Roads in that it has rails throughout the track. The track features a transparent multi-colored glass path and with star-shaped rails running throughout the entire course. Neon-light pictures of the eight playable characters, plus a portrait of a Boo and a Mushroom in the distance, float in the void. Moreover, a big three-dimensional smiling star lies in a curved section of the track. Roving Chomps slide through the course in reverse, tossing into the air any driver who touches them. The course returns in Mario Kart 8 as a heavily-revamped retro course, as the last course of the Lightning Cup.

Course layout
The twisting course starts with a huge drop followed by a gentle uphill stretch, passing through a rainbow ring. After the ring, there is a wide loop with constellations of Mario, Peach and Luigi on the loop's outside. Following the loop is a steady downward road that crosses over the steep drop where a Mushroom constellation can be seen. After the bridge is a corkscrew loop with a large star in the center and a Boo constellation at the corkscrew's outside. Racers turn around a corkscrew to reach a hairpin bend, where constellations of Donkey Kong and Yoshi are seen. Shortly after the hairpin bend is a short straightaway followed by a smaller drop and small uphill stretch. A Toad constellation can be seen after the drop as well as a wide right hand turn. A straightaway follows with a Wario constellation present, then follows a final looping section, where a Bowser constellation is seen. After the loop is a final straightaway to the finish.

Although this track may easily be both the easiest and longest Rainbow Road track due to the railings and length, racers can perform a large shortcut by making a timed hop to the left (or right, during Extra) during the drop at the beginning.

Mario Kart 8
Rainbow Road from Mario Kart 64 returns in Mario Kart 8, appearing as the final course in the Lightning Cup, which is similar to SNES Rainbow Road in Mario Kart 7. It has a large amount of changes to the original.

One of the biggest changes is the setting; just like Rainbow Road in the single-player mode of Mario Kart: Double Dash!!, it now takes place above a brightly lit city near a coast at night instead of a night sky background from the original. The road is now formed by colorful tiles with a golden frame, as opposed to the slightly transparent stripes from the original version, which is very similar to Rainbow Road from Super Mario Kart, while the starting area has a bronze diamond mosaic pattern. The tiles also appear to be made up of multi-coloured LEDs, based on its appearance and lighting.

Another change is the starting banner from the original being changed into a star-shaped ring; once players nearly reach the finish line, the gear-like goal ring will change its shape by expanding its star-shaped appendages wide and closing in upon itself. Also, there are now some nods to Super Mario Galaxy, such as the stationary star icon that appears in the original being replaced with a giant rotating Grand Star and the star rails are designed after Launch Stars. Also, most of the rails have been removed, adding that to the challenge. Another visual feature includes a flying train (that's based on an 0-4-0ST Porter Saddle Tank), with Toads riding them, who throw coins at certain areas, passing around the course. There are now fireworks around the course, with some that form the six playable characters that have been present since Super Mario Kart (Mario, Princess Peach, Luigi, Yoshi, Toad, and Bowser) that replace the neon-lit signs of the eight playable characters in Mario Kart 64 (the aforementioned six, Wario and Donkey Kong), and portrait of a Boo and a Mushroom from the original. Some of these fireworks take their appearance from stamps in Super Mario 3D World, though Yoshi's firework is based on artwork from Yoshi Topsy-Turvy.

The other big change is that the course has been altered to incorporate the game's hang-glider and anti-gravity mechanics. From a view above the course, the loop, in the middle of which sits the Grand Star, lies on the left side of the circuit instead of on the right side. At the gentle uphill stretch, which succeeds the steep slope, the rainbow ring has been replaced by an anti-gravity panel. The dip after the large U-Turn is now a full turn. Plus, instead of going through the course in a zigzag pattern in the original, the Chomps now bounce up and down, creating a wave on the track that can be used to perform tricks, which is similar, from Mario Kart 7, to how the Thwomps in SNES Rainbow Road create a wave on the track. As the Chomps land, the section they create a wave on will flash. Boost Pads and Launch Stars have been added.

Similar to Rainbow Road from Mario Kart 7, it is split into three sections, instead of three laps, and the last part before the finish line has also been slightly shortened, in order to cut down the length of the original, and on the final section, the chorus of the music now starts to play, instead of the beginning of the music. It is the first and only retro course to do this. The music of the course is also remixed, in a similar instrumentation to the Mario Kart 8 intro theme, with its main emphasis on trumpets, string sections, and saxophones, with a guitar solo in the chorus. This song, as with the entire soundtrack of Mario Kart 8, is live-recorded.

A map of the city, mentioning Toad Harbor, Moo Moo Meadows, and Wario's Gold Mine, can be seen in Super Bell Subway. Thus, this Rainbow Road is set above the first two, while in the case of Wario's Gold Mine, due to its location it's likely that the headquarters of the company running it are found in this city instead of the actual mine. Many of the companies whose advertising signs can be seen in the various tracks have their headquarters in this city. The railway lines that can be seen from this Rainbow Road, according to the map in Super Bell Subway, connect this city to many other places where tracks of Mario Kart 8 can be found.

F-Zero X
F-Zero X has a track that imitates this version of Rainbow Road, having the same map of the track, and following it basically. It is described as a "Psychedelic Experience". Parts, though not all of it, are rainbow-colored. Unlike the original, it has no barriers in certain areas, and the neon Mario series decor is missing - instead, it just has a plain black background with a watery blue bottom to which careless drivers may fall. In addition, when racing the track with the 64DD expansion features, the background song is a rock version of the Rainbow Road song heard in Mario Kart 64. There aren't any Chomps, but there are deadly minefields on certain sections of the track.

Official descriptions
Mario Kart 64 Instruction manual description: ''This course can be described in a word -- LONG, very long. It is simply the longest of all the courses. As the name indicates, the road is made of rainbow and it has a fantastic view of neon sculptures twinkling in the distance. The final course of the Special Cup, it is definitely worth seeing. It is advisable to slow down to avoid the Chomps that will attack.''

Trivia

 * In Mario Kart 64, the neon lights of Mario, the Mushroom, and Boo are the only ones that are animated.
 * Rainbow Road is one of only two non-SNES courses in the series that differ in lap count between its original and remade versions; the other is Baby Park.
 * These courses are both the longest and the shortest courses in the series.
 * In Mario Kart 8, this course, Rainbow Road, and the downloadables SNES Rainbow Road and  GCN Baby Park are the only courses in the game to have the map in different colors instead of just blue.
 * The map of its reappearance in Mario Kart 8, shown in the Prima guide, has two Boost Pads on the third section placed on the right side. However in the game, they are actually placed on the left side.
 * In Mario Kart 8, the placement of the first two boost pads on the steep slope and the first two launch stars varies every time the player enters the race on this course.
 * There is a possible reference between the flying train in the new version and the music in the original. The train might reference the fact that the first part of the music in the original version uses a synthesized instrument (computer generated) that sounds like a train whistle, more specifically, a pan pipe.