N64 Rainbow Road

Rainbow Road is the longest race course in Mario Kart 64 at 2,000 meters and requiring around two minutes per lap, making it the longest in the whole series. The track differs from the other Rainbow Roads in that it has rails throughout the track. The track features a transparent multi-colored path and with star-shaped rails running throughout the course. Neon-light pictures of the eight playable characters, plus a portrait of a Boo and a Mushroom in the distance, float in the void. Moreover, a big three-dimensional smiling star lies in a section of the track. Roving Chain Chomps slide through the course in reverse, tossing into the air any driver who touches them.

This course will return in Mario Kart 8, being the final course in the Lightning Cup, much similar to SNES Rainbow Road in Mario Kart 7. Though the layout is much the same, the course has been altered to incorporate the game's hang-glider and anti-gravity mechanics and like Rainbow Road from Mario Kart 7, it will be spilt into three sections, possibly to cut down the length of the original. Most of the star railings have also been removed. It takes place above a city, instead of the black background from the original, the neon-lit characters are replaced with fireworks that form the characters, a train can now be seen passing around the course and boost panels from the previous two Rainbow Roads have been added. The star icon that appeared in the original has been replaced with a Grand Star from ''Super Mario Galaxy.

Course layout
The twisting course starts with a huge drop followed by a gentle uphill stretch, passing through a rainbow ring. After the ring, racers behold the flashing neon portraits while run through a pigtail bridge, following then towards another shallower drop. Racers turn around a corkscrew to reach a hairpin bend, and then a twisting stretch to go back to the checked line finally.

Although this track may easily be the easiest Rainbow Road track due to the railings, racers can perform a large shortcut by making a timed hop to the left (or right, during Extra) during the drop at the beginning.

F-Zero X has a track that imitates this Rainbow Road, having the same map of the track, and following it basically. It is described as a "Psychedelic Experience". Parts, though not all of it, are rainbow-colored. Unlike the original, it has no barriers in certain areas, and the neon Mario series decor is missing - instead, it just has a plain black background with a watery blue bottom to which careless drivers may fall. In addition, when racing the track with the 64DD expansion features, the background song is a heavy metal version of the Rainbow Road song heard in Mario Kart 64. There aren't any Chain Chomps, because they are Mario-exclusive.

Instruction manual description
''This course can be described in a word -- LONG, very long. It is simply the longest of all the courses. As the name indicates, the road is made of rainbow and it has a fantastic view of neon sculptures twinkling in the distance. The final course of the Special Cup, it is definitely worth seeing. It is advisable to slow down to avoid the Chomps that will attack.''