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 * On the cover of New Super Mario Bros. Wii's game disc, Mario, Luigi, Blue Toad, and Yellow Toad are positioned in the same pattern as the buttons on the European and Japanese Super Nintendo Entertainment System controller.
 * In Bowser in the Sky, the pillars near the Warp Pipe to the Bowser battle have engraved markings of an 8-bit Mario fighting an 8-bit Bowser, likely a callback to the original Super Mario Bros.
 * In the mission Blasting through the Sand in the Dusty Dune Galaxy, the Eye Beamers on the third Speedy Sand Planet initially spawn inside the large planet at the center of the galaxy, only moving to the Speedy Sand Planet after the level is loaded.
 * Water Park's Aqua Cups ride plays the calliope music from the carousel in Big Boo's Haunt.