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 * Dr. Light, from the Mega Man series, not only created Mega Man, he also accidentally created a bad clone of Mario - the aptly named Wario.
 * The gigantic Bowser Crush Mecha-Koopa was originally created to squish flowers and scare butterflies, but was later used as a weapon against the Smithy Gang.
 * Clawdia Koopa is Bowser's wife, although her actual existence is questionable, as she was only mentioned in a joke.