Moo Moo

Cows are a species of animals on Mushroom World and also the real world. In The Super Mario Bros. Super Show! episode "Mario and the Beanstalk", Mario and Luigi had to get Gold Coins for the Mushroom Kingdom Orphanage, so they decided to sell Princess Toadstool's pet cow (the Royal Cow) to Dealin' Delbert. Delbert wanted to trade one of his many cows with Royal Cow, but the Mario Bros. wanted money. They instead traded Toadstool's Royal Cow for Garbanzo beans. Several Cows, with holes riddled throughout their bodies, later appeared as background characters in the episode "Koopenstein".

Cows can be seen in the background of a Mario Kart 64 and Mario Kart DS course named Moo Moo Farm. In Mario Kart: Double Dash!! ' s Mushroom City stage, a truck advertising this Moo Moo Farm course features a cow. Kamek mentioned a cow in Mario & Luigi: Partners in Time; on Yoshi's Island before his battle, Baby Bowser was eating too many Yoshi Cookies very fast and ended up choking. Cows are briefly mentioned in Paper Mario, as in the Chapter 4 Interlude, when Peach goes up to the milk, it says "Milk, fresh from the Cow!". Whilst viewing the child choke, Kamek suggested he drink some milk from an "evil cow". They recently appeared in Mario Kart Wii as in obstacle and background element of Moo Moo Meadows. It also was in the past stages of Moo Moo Farm.

Cows also appear in Mario Strikers Charged Football as course element in Thunder Island; these cows become dangerous objects that fall down from a cyclone and raze anybody throughout the field. Mario Kart Wii returns cows to the series, and this time, cows serve as obstacles in a course called Moo Moo Meadows. A truck in the Moonview Highway course, also has a picture of a cow printed on it with the text "Moo Moo Dairy". Cows have also made various appearances in the WarioWare Inc. series.