List of Mario & Luigi: Paper Jam pre-release and unused content

In the Nintendo Direct, the Kamek boss fight took place in Sunbeam Plains instead of Neo Bowser Castle. Screenshots also showed Koopa Troopas and Paper Koopa Troopas being fought in Sunbeam Plains. In the final version, they aren't encountered until Twinsy Tropics.

At E3, Battle Cards could seemingly be used as early as the Petey Piranha boss fight, where in the final game they are first used against Wendy and Roy. Also, the number over the cards (which shows the number of Star Points needed to use it) is written in a different color than in the final game (orange as opposed to yellow) and is about twice as small. Also in the demo, Paper Mario is able to create copies and perform another action in a single turn: this is normally only possible with a Star Rank-Up Bonus.

Paper Luigi was also once planned to be playable but was scrapped due to having four buttons to press making the game too difficult.