Mario & Sonic at the Rio 2016 Olympic Games (Nintendo 3DS)

Mario & Sonic at the Rio 2016 Olympic Games is a sports title for the Nintendo 3DS, which is the fifth installment on the Mario & Sonic series, along with the Wii U version. Like the other games, it takes place in the host city of the Olympics of the corresponding year, being Rio de Janeiro. This also has amiibo compatibility.

Characters
All playable characters from previous games in the series return, in addition to 20 new playable characters (10 new Mario characters and 10 new Sonic characters), for a current total of 41 playable characters. Similarly to the Nintendo 3DS version of Mario & Sonic at the London 2012 Olympic Games, characters are only playable in certain events, with the exceptions of Mario, Sonic, and Mii characters (who are playable in all events). All the other returning characters are exclusive to two events each, while the new characters are only available for one. Also, this is the first game in the series to feature unlockable playable characters (indicated by an *). Characters are unlocked by beating them in Road to Rio on the Plus version of the main event of that day.

Other

 * Miis (available in all events)

Supporting characters

 * Toads
 * Yoshis
 * Birdos
 * Chao
 * Egg Pawns

Non-playable characters

 * Shy Guys
 * Koopa Troopas
 * Goombas
 * Boos
 * Thwomps
 * Piranha Plants
 * Goomba Towers
 * Orbot
 * Cubot
 * Charmy Bee

Events
There are a total of 14 events in the game, each with two variants; a "Normal" version in which the events behave similarly to their real-life counterparts, and a "Plus" version where Mario and Sonic elements are incorporated into the event.
 * 100m Dash
 * 110m Hurdles
 * Table Tennis
 * Javelin Throw
 * BMX
 * Long Jump
 * Beach Volleyball
 * 100m Freestyle
 * Equestrian
 * Golf
 * Rhythmic Gymnastics: Hoop
 * Boxing
 * Archery
 * Football

Training Events
In the Road to Rio mode, the player can train by playing 30 events from the 3DS version of Mario & Sonic at the London 2012 Olympic Games. These events are:
 * Pole Vault
 * Keirin
 * 100m Backstroke
 * Hockey
 * Badminton (Singles)
 * Triple Jump
 * Shot Put
 * Synchronized Swimming (Team)
 * Trampoline
 * Synchronized Swimming (Duet)
 * 1000m Kayak (Singles)
 * 4 x 100m Relay
 * 1500m
 * Water Polo
 * 100m Breaststroke
 * Canoe Slalom (Pair)
 * Handball
 * Badminton (Doubles)
 * Double Trap
 * Rowing - Quadruple Sculls
 * Rings
 * Fencing - Epée
 * Platform Diving (Synchronized)
 * Sailing - 470 (Pair)
 * 25m Rapid Fire Pistol
 * Taekwondo
 * 10km Marathon Swim
 * Hammer Throw
 * Basketball
 * 3000m Steeplechase

amiibo
The game features amiibo functionality, but only with the Mario and Sonic amiibo. Using them will turn the Mario costume into the Gold Mario costume and the Sonic costume into the Super Sonic costume respectively.

Road to Rio
The game features a Road to Rio mode similar to the Adventure Tours in the DS version of Mario & Sonic at the Olympic Winter Games and the Castle Club in Mario Golf: World Tour, with the Mii challenging different characters to events. To train for the main events, the player must play a selection of events from the 3DS version of Mario & Sonic at the London 2012 Olympic Games. After playing a certain amount of rounds, the player will challenge a tower stage and then progress onto a new area. The mode is divided into two sections, accessed by joining the respective gyms: Mario's Story and Sonic's Story, with a special ending for completing both.

Each section is divided into 7 days with one main event happening on each day. Each section will have the players team up with one of two gyms and try to beat the opposing club. Along the way sub plots start and soon the two mascots are nowhere to be found.

Challenge Mode
The game includes a challenge mode similar to the Super Smash Bros. games and Kid Icarus: Uprising.

Marathon
The game has a special mode where based on the number of steps the player's Mii moves, going certain distances unlocks certain milestones.

Trivia

 * This is the first game in the Mario & Sonic series to feature the playable debut of characters, specifically Eggman Nega, Zazz, Zavok, and Pom Pom, with the latter being a playable, but not selectable supporting character.
 * This is the first handheld Mario & Sonic game where Sonic has text-based dialogue, and the first overall game where Nabbit has dialogue.
 * This is the first game in the series where the handheld version was released before the console version.
 * Each of the unlockable characters are playable in one event exclusive to that character with no overlaps and no events skipped.