Papa Mario

Mario and Luigi's father, presumably Mr. Mario or Papa Mario, is Mario and Luigi's father. He is a wide-set man, similar in frame to Mario. At the time of his sons' births, Mario and Luigi's father was living in a small mushroom-shaped house in the Mushroom Kingdom with Mama Mario, though it is not confirmed that they were married. Many other aspects of his life, including his current appearance, personality and residence, are unknown, and the few sources of information pertaining to Mario and Luigi's father are widely contradictory.

Yoshi's Island series
Mario and Luigi's father appeared at the end Super Mario World 2: Yoshi's Island, when he and his supposed wife found Baby Mario and Baby Luigi on their doorstep, who had been delivered by the stork. Unaware that his sons had just been rescued from the Koopa Troop by the Yoshis, Mr. Mario held Luigi aloft (Mama Mario had Mario), and entered the world of parenthood.

However, the babies were not yet out of danger, and were kidnapped once more by Kamek in the middle of the night during the events of Yoshi's Island DS, causing a great commotion in the Mario household. But while Papa and Mama Mario knew that their children were in peril this time, they were still powerless against the Koopas, and the Yoshis saved the day once again. The stork returned the babies, and although it was not the end of their adventures (as babies or as adults), their father has not made another in-game appearance (not counting the Game Boy Advance port of Yoshi's Island: Super Mario Advance 3).

Super Mario Amada series
Papa Mario first appeared in the Super Mario Amada series adaptation of Issunboshi. The events depicted in this anime greatly differ from those in the Yoshi and greater Mario series of video games (for example, Luigi does not appear at all). In this version, Mario was miraculously born in Papa and Mama Mario's household when a shooting star passed over their small dwelling. Mario was extremely tiny: much smaller than Papa Mario's hand. The couple raised the baby, but to their amazement the child remained tiny as he aged into adulthood. The parents were very protective of their son because of his size, keeping him near their house and away from potentially dangerous places like the nearby city. One day, Mario attempted to convince his parents that he was old enough to explore the world alone, despite his small size. Papa and Mama Mario eventually agreed with their son, allowing Mario to independently pursue his own adventures. Before Mario left for his first destination, Papa Mario fashioned a few objects to help his pint-sized son on his journey. Mario's father turned a small needle into a sword that matched Mario's size. He also manufactured a small jet-propelled boat using a common bowl and various mechanical parts for Mario to use. Lastly, Papa Mario fashioned a small piece of wood into a perfectly-sized oar to help Mario propel his boat (instead of using the rocket boosters). When everything was completed, Papa Mario carried his son and the boat to the river and placed them in the water, letting Mario finally live his life on his own.

Nintendo Comics System
Mr. Mario was pictured in the third panel of Family Album "The Early Years", which was released five years before Yoshi's Island, but is set later in Mario's life, when Mario was a toddler. In the comic, Mario is shown to have been meddling with his father's pipe, making it produce bubbles instead of smoke. Mario is pictured fleeing from from his irate father.

Relatives
In the games, Mr. Mario has two sons: Mario and Luigi. Their mother, Mama Mario, is presumed to be his wife. Earlier sources (such as Family Album "The Early Years" and the game Super Mario Bros.) implied that Mario was older than Luigi, however as of Yoshi's Island, they are usually recognized as twins.

The Super Mario Bros. Super Show! introduces many more members of the Mario family. Mr. Mario has a pair of nieces named Marianne and Luigeena, and two nephews called Mario Joe and Luigi Bob. As it is not revealed which sides of the family any of the relatives hail from, it is unknown whether Grandma Mia is Mr. Mario's mother or mother-in-law, and whether Aunt Luigeena is his sister or sister-in-law. The Adventures of Super Mario Bros. 3 mentions a second sister/sister-in-law named Aunt Maria.

Also, according to the Saturday Supercade ' s Donkey Kong segment, Pauline is Mario's niece, which would make Mr. Mario her grandfather. However, this contradicts the continuity of the Mario series, in which she is Mario's ex-girlfriend, and thus of no relation to his parents.