From the Super Mario Wiki
Super Mario series
Super Mario 64 / Super Mario 64 DS
They first appeared in Super Mario 64 and later in its remake Super Mario 64 DS. They act the same as Goombas; when in range they charge at Mario, and punching and stepping on their heads defeats them. They live in Hazy Maze Cave and outside Big Boo's Haunt. The only difference between Goombas is that they give three Coins instead of just one. But in the remake, their appearance changed drastically, taking the shape of their New Super Mario Bros. appearance.
New Super Mario Bros.
Scuttlebugs reappear in New Super Mario Bros., hanging down from webs in World 4-1. They will repeatedly move up and down, and can prove to be useful in giving some extra height by jumping off of them. They also appeared in in World 8-4, where they will ceaselessly drop down from the top of the stage in pairs of two. They detach from their webs almost immediately, and will drop onto the ground and give chase.
Super Mario Galaxy
Scuttle Bugs reappear in Super Mario Galaxy. These Scuttle Bugs are striped and, unlike their bright yellow cousins, mostly dark and dull in color. Some will hang from ceilings and overhangs by a thread of webbing. They appear mostly in the Space Junk Galaxy and Ghostly Galaxy, and make appearances in other galaxies and Bowser Jr.'s Airship Armada. They are found in large numbers along the path leading to Tarantox. The Prima Guide calls the jumping ones Spoings and the ones hanging from webs Spranglers.
The grounded Scuttle Bugs are orange and will only attack by jumping into the air and floating there for several seconds before falling back to the ground in hopes of landing on Mario. The kind that hangs from ceilings is purple and will swing back and forth on their threads like a pendulum in an attempt to damage the player. The grounded ones could be simply defeated by jumping on them or Spinning them, and the ones that hang from ceilings could be defeated by jumping on them or with two Spins. They would give a Coin or three Star Bits depending in how they are defeated.
Super Mario Galaxy 2
Scuttle Bugs reappear in Super Mario Galaxy 2, with the same appearance and behavior from the predecessor. However, unlike the previous game, they are rarer, only appearing in a few galaxies. They appeared in Yoshi Star Galaxy, Tall Trunk Galaxy, and Flip-Out Galaxy.
New Super Mario Bros. 2
Scuttle Bugs appear in the Nintendo 3DS game New Super Mario Bros. 2. They hang from webs, just like their New Super Mario Bros. incarnations. However, their appearance have changed; instead of being yellow with three black stripes and having black heads, they are yellow with two purple stripes and heads, as well as having white pincers. They appeared in World 3-3 and World 3-Cannon.
Mario Party series
Mario Party 7
In the Mario Party 7 minigame Spider Stomp, Scuttle Bugs appear, along with their probable ruler, a big Scuttle Bug. However, they are simply known as "spiders" in the game and can shoot web projectiles. The red machine in Hop-O-Matic 4000 resembles a Scuttle Bug.
Mario Party DS
Scuttle Bugs appear also in Mario Party DS. It appears in only one minigame: a 2-Vs.-2 minigame named Crazy Crosshairs. Both teams must hit the Scuttle Bugs with some toy tanks on a spiderweb. This minigame also introduce Gold Scuttle Bugs, giving the team 3 points instead of one if hit. In the same game, the player can win a Scuttle Bug Rival Badge and a Scuttle Bug Trophy by getting MP Points, and look at them in Gallery Mode. But here, the character figure information on the Scuttle Bug refers to the Plunger-like feet as shoes.
Paper Mario: Sticker Star
Scuttle Bugs make their Paper Mario debut in Paper Mario: Sticker Star. In the game, they are referred to as Scuttlebugs. They are only found in World 3, with their appearance the same as from New Super Mario Bros.. Many walk around on the ground while others are attached to the ceiling using thread-like webs. If Mario battles a Scuttlebug on the ground, the enemy can either slam into Mario or shoot a web at him. If a hanging Scuttlebug is battled, it can only slam into Mario, but with a much higher damage ratio. The Scuttlebugs can also change their position on their strings to avoid hammer and ground attacks, similar to that of Piders and Arantulas from Paper Mario: The Thousand-Year Door. Scuttlebugs hold a Wiggler Segment hostage in Rustle Burrow, an area with most of its inhabitants being Scuttlebugs.
There are also Mini Scuttle Bugs that cannot be battled, but will chase Mario if he gets close to them. If a Mini Scuttle Bug touches Mario, he will jump lower and move slower until the Mini Scuttle Bug is removed, very similar to Micro Goombas, that have the same purpose in other games. Mini Scuttle Bugs can be defeated by getting hit with a hammer, touched by Invincible Mario, or being jumped on. Killing one will result in getting a Coin or maybe a Jump sticker. If a Mini Scuttle Bug is defeated with a Super Star, only a Coin could be dropped. A Big Scuttlebug also appears as a mini-boss.
Mario Kart Arcade GP DX
Scuttle Bugs also appear as items in Mario Kart Arcade GP DX, the player can use them to hit other racers with spiderwebs.
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