From the Super Mario Wiki
The Sapphire Cup is the second out of four cups in Donkey Kong Barrel Blast. It is unlocked when the Topaz Cup is beaten. It consists of four courses which include Scorching Canyon, DK Jungle Falls, Shimmering Sea, and Mammoth Glacier. The racer that places third place stands on a small tree. The racer in second place stands on a bigger tree. The racer that places first stands on the biggest tree.