Mario Bros. may refer to:
- Mario Bros., the arcade game released before the Super Mario Bros. NES title.
- Mario Bros., the Game & Watch title which featured Mario and Luigi working together in a factory. It was later re-created for Game & Watch Gallery 3 and Game & Watch Gallery 4.
- Mario Bros., a stage in Super Smash Bros. Brawl.
- Super Mario Bros., one of the first Mario platformers, first released for the Nintendo Entertainment System.
- Mario Brothers, a microgame from WarioWare: Twisted!
- The Super Mario Bros. live-action film, featuring an alternate storyline and starring Bob Hoskins and John Leguizamo as Mario and Luigi respectively.
- Mario and Luigi, the heroes of the Mushroom Kingdom who are often called the "Mario Bros." or the "Super Mario Bros."
- Baby Mario and Baby Luigi, the baby versions of Mario and Luigi, respectively.
- Luigi Bros., a remake of Mario Bros. starring Luigi that's included in Super Mario 3D World.
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