“Everyone deserves what they've earned! And I've earned this!”
In the beginning of the movie, Lena seems to be enjoying the high life with Koopa. However, when he finds Princess Daisy, she becomes exceedingly jealous and feels that everything that she's worked for, to be by Koopa's side and rule the world with him, have been dashed to pieces. After she finds the piece of meteorite needed to merge the two worlds, she comes back to him, but he spurns her. Lena then decides to take matters into her own hands and attempts to merge the two worlds on her own. She then arrives at Daisy's office (which had formerly been her own office) and decides to "help" Daisy in leaving by trying to kill her. However, her attempted assassination went awry after Yoshi, the pet of the royal family, acted in Daisy's defense and proceeded to maul Lena, using its tongue to drag her to him, although she retaliated by stabbing him, wounding him. Koopa eventually figures out her deception after learning from one of his officers of a status report regarding the process of the invasion that was "authorized" by him without his knowledge, and arrests her. Later, however, during Koopa's fight with Mario, he drops the meteorite and she grabs it again, although she ended up electrocuted due to the railing breaking and falling onto an electric network. She finds the meteor and tries to put the piece back in. Her plans prove deadly, and as she puts the meteor piece back in the with larger meteor, she is electrocuted and immediately reduced to a skeleton that gets stuck to the rock wall.
While gloating about how she had succeeded in acquiring the meteorite, she states that "the universe is hers," which implies that she most likely wanted to use the meteor to rule the entire universe, without any regard to her people.
Lena was portrayed by Fiona Shaw.