From the Super Mario Wiki
This gigantic Blooper blocks Mario, Bowser, and Princess Peach from moving on to Fort Francis. There are a couple options for offense against this monster. The player may use Thudley to ground-pound the tentacles or use Bowser's fire breath to sear them. The fiery-breath may be the safer choice, but takes longer. Slamming or burning the blue tentacles makes the more-sensitive red tentacles bob upward with more frequency. It is a good idea to slam exposed blue tentacles across the bottom of the screen, but the player will lose Heart Points if they miss.
Defeating Big Blooper doesn't earn the player any points, and the giant squid isn't killed by the encounter. If the player enters the area where the giant Blooper's tentacles come out of the floor and ceiling, Big Blooper can be tattled this way using Tippi or Tiptron.
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