From the Super Mario Wiki
Big Bessie is a cannon that appears in Donkey Kong Country 3: Dixie Kong's Double Trouble!, and is owned by Bazooka on the island of Mekanos.
Bazooka is somewhat disappointed, because he hasn't been able to fire up Big Bessie in years. However, should Dixie and Kiddy Kong bring Bazooka the Bowling Ball that they can receive from Blue, he will be able to use it as ammunition for Big Bessie. By doing so, the Kongs will climb into Big Bessie's barrel, which will blast them to a Banana Bird Cave suspended on a crane. Should the Kongs need to return to the cave, Bazooka will permit them to use Big Bessie again.